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Hepatic micronucleus assay for the assessment of genotoxic responses in fish

✍ Scribed by Rao, Salem S. ;Nehell, T. ;Metcalfe, C. D.

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
382 KB
Volume
11
Category
Article
ISSN
1053-4725

No coin nor oath required. For personal study only.

✦ Synopsis

This procedure is to be used to detect responses to genotoxic agents in fish through quantification of the number of micronuclei in hepatocytes of the teleost liver.

SCOPE

This procedure is applicable to the following:

  1. Fish sampled from waters contaminated by discharges of industrial effluents, in-place pollutants, surface runoff, and other sources of chemical contamination. 2. Fish exposed in the laboratory to chemical or physical genotoxic agents by an appropriate dosing method (e.g., intraperitoneal injection, gavage, or water borne exposures).

The hepatic micronucleus assay may be a

valuable in uiuo testing method or environmental biomonitoring tool for the assessment of the toxic impact of environmental genotoxic agents and carcinogens.

PRINCIPLE

Genotoxic agents and carcinogens in the environment initiate their toxic impacts on organisms at the sub-cellular level. Some of these 4.0 4.1. 4.1 4.1 4.1

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