Hepatic ischemia-reperfusion injury modification during liver surgery in rats: Pretreatment with nifedipine or misoprostol
✍ Scribed by Hardy, Kenneth J. ;Tancheroen, Somsit ;Shulkes, Arthur
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1995
- Tongue
- English
- Weight
- 741 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1074-3022
No coin nor oath required. For personal study only.
✦ Synopsis
The aim of the study was to determine if pretreatment with misoprostol (a prostaglandin analogue) or nifedipine (a calcium antagonist), known protectants of the whole liver, would ameliorate the ischemia-reperfusion injury (IRI) of resected liver associated with vascular occlusion. Male Wistar rats were allocated to 5 groups (n = 20 each group): sham-operated, liver resection only, liver resection plus pretreatment with 0.1 mg/kg misoprostol, 10 mg/kg, or 2 mg/kg nifedipine during the 3 days before IRI with liver resection. Fifteen percent of the liver was made ischemic by 30minute continuous vascular occlusion, and the remaining 85% nonischemic liver was resected. The model was designed to have survival of the rats so that liver function could be studied over 3 weeks. Seventeen of 20 control resection rats survived indicating a suitable model for study. The bilirubin level was reduced by 25% on postoperative days 3 through 23 with misoprostol. The ascular isolation of the liver is created during V liver surgery to reduce blood loss, because volume of blood loss relates to mortality and morbid-it^.'-^ However, this vascular occlusion produces a warm ischemia-reperfusion injury (IN) of the liver. A healthy whole liver can withstand total warm ischemia for about 1 hour, but the safe ischemia time with a major resection is not known; nor is it known if medications that protect against the IRI in the whole liver will have the same beneficial effects in the regenerating reduced volume liver. Our studies set out to address this question. The IRI involves hepatocytes, Kupffer cells (KC), endothelial cells, and biliary epithelial cells. Preservation of liver function depends on maintaining all four.
The damage from ischemia is lack of oxygen,