Hemostasis and thrombosis : methods and protocols

✍ Scribed by Favaloro, Emmanuel J.; Lippi, Giuseppe

• Year: 2017
• Tongue: English
• Leaves: 550
• Series: Methods in molecular biology 1646; Springer protocols
• Category: Library

✦ Synopsis

This collection thoroughly explores the dynamic and ever-developing field of hemostasis and thrombosis diagnostics and research. After an introductory section covering the basics and preanalytical issues, the book continues with in-depth sections that explore how to get the best outcomes from routine coagulation and specialized hemostasis assays, thrombophilia-related techniques, investigations into bleeding disorders, as well as performance of global assays of hemostasis, and finally post-analytical issues in hemostasis and thrombosis testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Hemostasis and Thrombosis: Methods and Protocols serves as an ideal resource for researchers and diagnostic laboratories seeking expert guidance and working to identify the best methodologies to pursue hemostasis and thrombosis testing.

✦ Table of Contents

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
Overview of Hemostasis and Thrombosis and Contribution of Laboratory Testing to Diagnosis and Management of Hemostasis and Thrombosis Disorders (Roslyn A. Bonar, Giuseppe Lippi, Emmanuel J. Favaloro)....Pages 3-27
Preanalytical Issues in Hemostasis and Thrombosis Testing (Giuseppe Lippi, Emmanuel J. Favaloro)....Pages 29-42
The Interface between Immunotransfusion and Hemostasis and Thrombosis Testing (Massimo Franchini)....Pages 43-56
Front Matter ....Pages 57-57
Optimizing the Verification of Mean Normal Prothrombin Time (MNPT) and International Sensitivity Index (ISI) for Accurate Conversion of Prothrombin Time (PT) to International Normalized Ratio (INR) (Emmanuel J. Favaloro)....Pages 59-74
Performance of Activated Partial Thromboplastin Time (APTT): Determining Reagent Sensitivity to Factor Deficiencies, Heparin, and Lupus Anticoagulants (Geoffrey Kershaw)....Pages 75-83
Performance and Interpretation of Mixing Tests in Coagulation (Geoffrey Kershaw)....Pages 85-90
D-Dimer Testing: Laboratory Aspects and Current Issues (Jecko Thachil, Giuseppe Lippi, Emmanuel J. Favaloro)....Pages 91-104
Determination of Fibrinogen and Thrombin Time (TT) (Anetta Undas)....Pages 105-110
Front Matter ....Pages 111-111
An Overview of Thrombophilia and Associated Laboratory Testing (Martina Montagnana, Giuseppe Lippi, Elisa Danese)....Pages 113-135
Laboratory Testing for Activated Protein C Resistance (APCR) (Soma Mohammed, Emmanuel J. Favaloro)....Pages 137-143
Assessment of Hereditary Thrombophilia: Performance of Protein C (PC) Testing (Richard A. Marlar, Jana N. Gausman)....Pages 145-151
Assessment of Hereditary Thrombophilia: Performance of Protein S (PS) Testing (Richard A. Marlar, Jana N. Gausman)....Pages 153-160
Assessment of Hereditary Thrombophilia: Performance of Antithrombin (AT) Testing (Jana N. Gausman, Richard A. Marlar)....Pages 161-167
Lupus Anticoagulant Testing: Diluted Russell Viper Venom Time (dRVVT) (Vittorio Pengo, Elisa Bison, Alessandra Banzato, Giacomo Zoppellaro, Seena Padayattil Jose, Gentian Denas)....Pages 169-176
Lupus Anticoagulant Testing: Activated Partial Thromboplastin Time (APTT) and Silica Clotting Time (SCT) (Armando Tripodi, Veena Chantarangkul)....Pages 177-183
Solid Phase Immunoassay for the Detection of Anticardiolipin Antibodies (Rohan Willis, Elizabeth Papalardo, E. Nigel Harris)....Pages 185-199
Solid Phase Immunoassay for the Detection of Anti-β2 Glycoprotein I Antibodies (Rohan Willis, Elizabeth Papalardo, E. Nigel Harris)....Pages 201-215
Measuring Direct Oral Anticoagulants (Robert C. Gosselin, Jonathan Douxfils)....Pages 217-225
Laboratory Testing Protocols for Heparin-Induced Thrombocytopenia (HIT) Testing (Kun Kan Edwin Lau, Soma Mohammed, Leonardo Pasalic, Emmanuel J. Favaloro)....Pages 227-243
Front Matter ....Pages 245-245
One-Stage Factor VIII Assays (Elizabeth Duncan, Susan Rodgers)....Pages 247-263
Chromogenic Factor VIII Assays for Improved Diagnosis of Hemophilia A (Susan Rodgers, Elizabeth Duncan)....Pages 265-276
Assessment of Factor XIII (László Muszbek, Éva Katona, Adrienne Kerényi)....Pages 277-293
Detection and Measurement of Factor Inhibitors (Geoffrey Kershaw)....Pages 295-304
Platelet Function Tests: Preanalytical Variables, Clinical Utility, Advantages, and Disadvantages (Anne-Mette Hvas, Erik Lerkevang Grove)....Pages 305-320
Platelet Function Analyzed by Light Transmission Aggregometry (Anne-Mette Hvas, Emmanuel J. Favaloro)....Pages 321-331
Whole Blood Platelet Aggregometry (George A. Fritsma, David L. McGlasson)....Pages 333-347
Assessment of Platelet Function in Whole Blood by Flow Cytometry (Leonardo Pasalic)....Pages 349-367
Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood (Leonardo Pasalic, Gabrielle J. Pennings, David Connor, Heather Campbell, Leonard Kritharides, Vivien M. Chen)....Pages 369-389
Diagnosis or Exclusion of von Willebrand Disease Using Laboratory Testing (Emmanuel J. Favaloro)....Pages 391-402
Laboratory Testing for von Willebrand Factor Antigen (VWF:Ag) (Emmanuel J. Favaloro, Soma Mohammed, Jürgen Patzke)....Pages 403-416
Laboratory Testing for von Willebrand Factor Collagen Binding (VWF:CB) (Emmanuel J. Favaloro, Soma Mohammed)....Pages 417-433
Laboratory Testing for von Willebrand Factor Ristocetin Cofactor (VWF:RCo) (Soma Mohammed, Emmanuel J. Favaloro)....Pages 435-451
Laboratory Testing for von Willebrand Factor Activity by Glycoprotein Ib Binding Assays (VWF:GPIb) (Jürgen Patzke, Emmanuel J. Favaloro)....Pages 453-460
Laboratory Testing for von Willebrand Factor: Factor VIII Binding (for 2N VWD) (Soma Mohammed, Emmanuel J. Favaloro)....Pages 461-472
Ristocetin-Induced Platelet Aggregation (RIPA) and RIPA Mixing Studies (Juan Pablo Frontroth, Emmanuel J. Favaloro)....Pages 473-494
Laboratory Testing for Von Willebrand Factor Multimers (Susan Oliver, Kun Kan Edwin Lau, Kent Chapman, Emmanuel J. Favaloro)....Pages 495-511
Front Matter ....Pages 513-513
Thrombin Generation Assays (TGAs) (Gian Luca Salvagno, Erik Berntorp)....Pages 515-522
The Overall Hemostatic Potential (OHP) Assay (Jennifer Curnow)....Pages 523-531
Thromboelastography (TEG) (Maha Othman, Harmanpreet Kaur)....Pages 533-543
Post-analytical Issues in Hemostasis and Thrombosis Testing (Emmanuel J. Favaloro, Giuseppe Lippi)....Pages 545-559
Back Matter ....Pages 561-565

✦ Subjects

HA