Hemorrhagic Fever Viruses: Methods and Protocols

✍ Scribed by Maria S. Salvato (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 416
• Series: Methods in Molecular Biology 1604
• Edition: 1
• Category: Library

✦ Synopsis

This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection.

✦ Table of Contents

Front Matter ....Pages i-xvi
Front Matter ....Pages 1-1
Global Spread of Hemorrhagic Fever Viruses: Predicting Pandemics (Jean-Paul Gonzalez, Marc Souris, Willy Valdivia-Granda)....Pages 3-31
An Approach to the Identification and Phylogenetic Analysis of Emerging and Hemorrhagic Fever Viruses (Francisco J. Díaz, Luis E. Paternina, Juan David Rodas)....Pages 33-41
Preliminary Classification of Novel Hemorrhagic Fever-Causing Viruses Using Sequence-Based PAirwise Sequence Comparison (PASC) Analysis (Yīmíng Bào, Jens H. Kuhn)....Pages 43-53
Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology (Carolina Montoya-Ruiz, Juan David Rodas)....Pages 55-78
Diagnostics for Lassa Fever: Detecting Host Antibody Responses (Maria S. Salvato, Igor S. Lukashevich, Sandra Medina-Moreno, Juan Carlos Zapata)....Pages 79-88
Sampling Design and Mosquito Trapping for Surveillance of Arboviral Activity (Luís E. Paternina, Juan David Rodas)....Pages 89-100
Epidemiological Surveillance of Rodent-Borne Viruses (Roboviruses) (Juan David Rodas, Andrés F. Londoño, Sergio Solari)....Pages 101-109
Front Matter ....Pages 111-111
Entry Studies of New World Arenaviruses (María Guadalupe Martínez, María Belén Forlenza, Nélida A. Candurra, Sandra M. Cordo)....Pages 113-133
Studies of Lassa Virus Cell Entry (Antonella Pasquato, Antonio Herrador Fernandez, Stefan Kunz)....Pages 135-155
A Cell-Cell Fusion Assay to Assess Arenavirus Envelope Glycoprotein Membrane-Fusion Activity (Joanne York, Jack H. Nunberg)....Pages 157-167
Assays to Assess Arenaviral Glycoprotein Function (Junjie Shao, Xiaoying Liu, Yuying Liang, Hinh Ly)....Pages 169-178
Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus (Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Dong, Hinh Ly)....Pages 179-188
Assays to Demonstrate the Roles of Arenaviral Nucleoproteins (NPs) in Viral RNA Synthesis and in Suppressing Type I Interferon (Qinfeng Huang, Junjie Shao, Yuying Liang, Hinh Ly)....Pages 189-200
Intracellular Detection of Viral Transcription and Replication Using RNA FISH (Michael E. Lindquist, Connie S. Schmaljohn)....Pages 201-207
Hemorrhagic Fever Virus Budding Studies (Ronald N. Harty)....Pages 209-215
Roles of Arenavirus Z Protein in Mediating Virion Budding, Viral Transcription-Inhibition and Interferon-Beta Suppression (Junjie Shao, Yuying Liang, Hinh Ly)....Pages 217-227
Structure–Function Assays for Crimean–Congo Hemorrhagic Fever Virus Polymerase (Marko Zivcec)....Pages 229-235
Minigenome Systems for Filoviruses (Thomas Hoenen)....Pages 237-245
Establishment of Bisegmented and Trisegmented Reverse Genetics Systems to Generate Recombinant Pichindé Viruses (Rekha Dhanwani, Qinfeng Huang, Shuiyun Lan, Yanqing Zhou, Junjie Shao, Yuying Liang et al.)....Pages 247-253
Front Matter ....Pages 255-255
Murine Models for Viral Hemorrhagic Fever (Rosana Gonzalez-Quintial, Roberto Baccala)....Pages 257-267
Testing Experimental Therapies in a Guinea Pig Model for Hemorrhagic Fever (Gary Wong, Yuhai Bi, Gary Kobinger, George F. Gao, Xiangguo Qiu)....Pages 269-278
A Primate Model for Viral Hemorrhagic Fever (Maria S. Salvato, Igor S. Lukashevich, Yida Yang, Sandra Medina-Moreno, Mahmoud Djavani, Joseph Bryant et al.)....Pages 279-290
A Primary Human Liver Cell Culture Model for Hemorrhagic Fever Viruses (Mahmoud Djavani)....Pages 291-302
Front Matter ....Pages 303-303
Protocol for the Production of a Vaccine Against Argentinian Hemorrhagic Fever (Ana María Ambrosio, Mauricio Andrés Mariani, Andrea Soledad Maiza, Graciela Susana Gamboa, Sebastián Edgardo Fossa, Alejando Javier Bottale)....Pages 305-329
Detection of Virus-Antibody Immune Complexes in Secondary Dengue Virus Infection (Meng Ling Moi, Tomohiko Takasaki, Ichiro Kurane)....Pages 331-337
Future Approaches to DNA Vaccination Against Hemorrhagic Fever Viruses (John J. Suschak, Connie S. Schmaljohn)....Pages 339-348
Front Matter ....Pages 349-349
Identifying Restriction Factors for Hemorrhagic Fever Viruses: Dengue and Junín (Federico Giovannoni, Jose Rafael Peña Cárcamo, María Laura Morell, Sandra Myriam Cordo, Cybele C. García)....Pages 351-370
Determining the Virus Life-Cycle Stage Blocked by an Antiviral (Claudia S. Sepúlveda, Cybele C. García, Elsa B. Damonte)....Pages 371-392
Retrovirus-Based Surrogate Systems for BSL-2 High-Throughput Screening of Antivirals Targeting BSL-3/4 Hemorrhagic Fever-Causing Viruses (Sheli R. Radoshitzky, Veronica Soloveva, Dima Gharaibeh, Jens H. Kuhn, Sina Bavari)....Pages 393-403
Protocols to Assess Coagulation Following In Vitro Infection with Hemorrhagic Fever Viruses (Melissa L. Tursiella, Shannon L. Taylor, Connie S. Schmaljohn)....Pages 405-417
Back Matter ....Pages 419-425

✦ Subjects

Immunology