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Hemocyanin-antibody labeling of rhodopsin in mouse retina for a scanning electron microscope study

✍ Scribed by Jan, Lily Yeh ;Revel, Jean-Paul

Publisher
Wiley (John Wiley & Sons)
Year
1975
Tongue
English
Weight
379 KB
Volume
3
Category
Article
ISSN
0091-7419

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✦ Synopsis

Abstract

To reveal the presence of rhodopsin on the surface of the mouse retina, a scanning electron microscope study of the immunolabeling of rhodopsin was attempted. The glutaraldehyde‐fixed mouse retina was treated first with rabbit antibodies specific against bovine rhodopsin and then with hemocynin‐labeled goat antibodies specific against rabbit antibody. The distribution of hemocyanin label on mouse retina and the technique used for labeling are discussed.

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