Hemmung des Sialinsäureeinbaus in Ganglioside und Glykoproteine des Kükenhirns durch Chlorpromazin

✍ Scribed by H. Rösner

• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 691 KB
• Volume: 48
• Category: Article
• ISSN: 0033-3158
• DOI: 10.1007/bf00423302

✦ Synopsis

Three-days-old White Leghorn chickens were used to study the effect of i.p.-injected chlorpromazine (CP, 2.5 or 5 mg/animal) on the incorporation of N-(3H)-acetylmannosamine into glycoprotein-and ganglioside-bound sialic acid and 14Chistidine into the proteins of the cerebrum, the optic lobes, and the cerebellum after incorporation periods of 1-24 h. All experiments were performed under constant temperature conditions.

1. The incorporation of 14C..histidine into the proteins of the optic lobes was not influenced by CP.

2. Within 24 h in the optic lobes neither the concentration of particle-bound sialic acid (~tg sialic acid/ mg protein) nor the content of radioactive-labeled sialic acid precursor was affected by the drug.

3. The rate of incorporation of labeled sialic acid into glycoproteins and gangliosides, however, was up to 6 h after the injection of the precursor much lower in the CP-treated animals than in controls. This may indicate, that the turnover of sialo-compounds was diminished by CP.

4. The inhibition was the most intensive in the cerebrum (up to 80 %), lower in the optic lobes (up to 70 %), and only small in the cerebellum (up to 20 %), but was always the same for glycoproteins and gangliosides.

The different inhibition rates in the different structures may indicate a correlation between the pharmacologic, sedative effects of the drug and the diminished turnover of sialo compounds.