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Hemicellulosic polymers from the cell walls of beeswing wheat bran: Part I, polymers solubilised by alkali at 2°

✍ Scribed by M.Susan DuPont; Robert R. Selvendran


Book ID
102992345
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
1000 KB
Volume
163
Category
Article
ISSN
0008-6215

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✦ Synopsis


Cell-wall material from beeswing wheat bran was sequentially extracted with 0.05~ NaOH at 2', M KOH at 2" and 20", and 4~ KOH at 20" followed by delignification and further extraction with M and 4~ KOH at 20", to leave the cr-cellulose residue which contained a significant amount of arabinoxylan. The hemicellulosic polymers solubilised by M KOH at 2", which represented -20% of the dry weight of the cell walls, were fractionated by graded precipitation with alcohol prior to anionexchange chromatography and then subjected to methylation analysis. The major polymers were closely related, highly branched arabinoxylans, slightly branched arabinoxylans, and arabinoxylans in close association with xyloglucans (arabinoxylan-xyloglucan complexes); the arabinoxylans were acidic and were associated with various amounts of phenolics. The various polymers exhibit heterogeneity, and phenolic ester and phenolic ether cross-links play a major role in the architecture of the cell walls.