Hemicellulosic contamination of acid detergent residues and their replacement by cellulose residues in cell wall analysis
✍ Scribed by Ian M. Morrison
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 427 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Abstract
The carbohydrate compositions of acid detergent residues, prepared from 26 plant samples, ranging from fruits, vegetables, legumes, forages and woods, were determined and up to 15.4% of the sugar units present were neutral sugars other than D‐glucose and derived from hemicelluloses. Similar analyses on cellulose residues, prepared by the acetic acid‐nitric acid method, showed up to 9.6% of these residues were derived from hemicellulosic contaminants. These results suggest that care should be taken before using acid detergent residue values to calculate the cellulose and hemicellulose content of plant materials. It is observed that more accurate results will be obtained by using values for cellulose residues and this method is recommended even though it is not absolutely accurate, particularly when analysing plant materials with a high cell wall content.