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Hematoporphyrin derivative fluorescence: Photographic techniques for the localization of malignant tissue

✍ Scribed by Raymond J. Lanzafame; David W. Rogers; John O. Nairn; Ralph P. Pennino; J. Raymond Hinshaw; Jeffrey R. Blackman


Book ID
102932986
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
853 KB
Volume
6
Category
Article
ISSN
0196-8092

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✦ Synopsis


Three simple methods for the detection of malignancy by the photography of hernatoporphyrin derivative (HPD) fluorescence are presented. Two methods employ a single lens reflex camera with macrolens and Kodak high-speed Ektachrome (EL 400) film. Both employ a Corning Glass #3482 filter as a barrier on the lens. In method I. a single Kodak Wratten #39 filter is placed over a fluorescent "black light" source (two General Electric F20T12BL 20-W bulbs). In method 11, twin electronic flash units (5,500 BCPS unfiltered) with a double thickness of Kodak Wratten #39 filter over them provide the light of excitation. Method 111 employs an adapted Polaroid@ SLR 680E Sun camera with two electronic flash units (5,500 BCPS, total unfiltered) rigged with a slave trigger. A Corning Glass #3482 filter is placed on the lens to act as a barrier. and two filters of Kodak Wratten #39 glass are placed on each flash unit. Photographs are taken in a darkened room.


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Laser excitation of hematoporphyrin derivatives (HPD) localizing in tumors of the tracheobronchial tree and bladder is useful in the identification and treatment of those tumors. A comparable utility for HPD in the endoscopic localization of colonic tumors may be possible. In this study the ability