Helium–neon laser in viability of random skin flap in rats

✍ Scribed by Carlos E. Pinfildi; Richard E. Liebano; Bernardo S. Hochman; Lydia M. Ferreira

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 168 KB
• Volume: 37
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.20190

✦ Synopsis

Background and objectives:

The purpose of this study was to determine the role of helium-neon (he-ne) laser random skin flap viability in rats.

Study design/materials and methods:

Experimentally controlled randomized study. forty-eight wistar-epm rats were used, weighed, and divided into 4 groups with 12 rats each. the random skin flap was performed measuring 10 x 4 cm, with a plastic sheet interposed between the flap and the donor site. the group 1 (control) underwent sham irradiation with he-ne laser. the group 2 was submitted to laser irradiation, using the punctual contact technique on the skin flap surface. the group 3 was submitted to laser irradiation surrounding the skin flap, and the group 4 was submitted to laser irradiation both on the skin flap surface and around it. the experimental groups were submitted to he-ne laser irradiation with 3 j/cm(2) energy density immediately after the surgery and for the four subsequent days. the percentage of necrotic area of the four groups was calculated at the 7th post-operative day, through a paper-template method.

Results:

Group 1 reached an average necrotic area of 48.86%; group 2, 38.67%; group 3, 35.34%; and group 4, 22.61%. after the statistic analysis, results showed that all experimental groups reached statistically significant values when compared to the control group, and group 4 was the best one, when compared to all groups of this study (p<0.001).

Conclusion:

The he-ne laser irradiation was efficient to increase random skin flap viability in rats.