Heavy metal induced DNA damage in plant measured by single cell gel electrophoresis
β Scribed by Li Shaoshan; Zhigang Nie; Yan Wang
- Book ID
- 119380098
- Publisher
- Elsevier Science
- Year
- 2008
- Tongue
- English
- Weight
- 85 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0378-4274
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The use of single cell gel electrophoresis (SCGE) has recently been applied to plant systems. We optimized the experimental conditions for SCGE analysis using nuclei isolated from different tissues of intact plants. Concentration-response curves of genomic DNA migration were analyzed in intact plant
Human lymphocytes from a cell culture were irradiated with laser pulses of an Xe-Cl excimer laser (308 nm) or a dye laser at wavelengths from 312 to 640 nm. After conversion of photoinduced DNA damage into DNA strand breaks the "comet assay" (single-cell gel electrophoresis) was used to determine th