𝔖 Bobbio Scriptorium
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Heavy-meromyosin-decoration of microfilaments fromMougeotiaprotoplasts

✍ Scribed by Karin Klein; Gottfried Wagner; Michael R. Blatt

Book ID
104751822
Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
561 KB
Volume
150
Category
Article
ISSN
0032-0935

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✦ Synopsis

The cell wall of the green alga Mougeotia was enzymatically digested by macerase and cellulysin. Released protoplasts were spread on poly-L-ornithine, formvar-carbon-coated grids, and cell fragments were collected for structural characterization. Large numbers of 5-7 nm filaments are seen which may be decorated with heavy meromyosin (HMM), a digest product of muscle myosin that binds specifically to actin, supporting the hypothesis that the phytochrome-mediated chloroplast movements in these algae are driven by a contractile complex of actomyosin.

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When statocytes of cress root-caps were incubated in an extraction medium containing phalloidin, bundles of microfilaments (6.6 +/- 2.4 nm in diameter) were found in the cortical cytoplasm. The inclusion of heavy-meromyosin in this medium demonstrated an arrowhead pattern on the filaments indicative