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Heats of thermally induced helix–coil transitions of DNA in aqueous solution

✍ Scribed by D. D. F. Shiao; J. M. Sturtevant

Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
432 KB
Volume
12
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Thermal denaturation of calf thymus DNA at both alkaline and neutral pH values was studied by differential scanning calorimetry. It was shown that the dependence of the enthalpy of transition on pH and salt concentration could be accounted for on the basis of a heat capacity change of +40 cal deg^−1^(base pair)^−1^. In the pH range between 10.3 and 11.3, a release of 0.6 proton per base pair was calculated from the pH dependence of the melting temperature. The heat effect associated with the release of this proton was calculated to be 5 kcal mole^−1^.

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