Heat shock increases antigenic peptide generation but decreases antigen presentation

✍ Scribed by Elsa Pépin; Christian L. Villiers; Françise M. Gabert; Vincent A. Serra; Patrice N. Marche; Maurice G. Colomb

• Book ID: 102162959
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 471 KB
• Volume: 26
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830261220

✦ Synopsis

Heat shock increases antigenic peptide generation but decreases antigen presentation

The heat shock response is a universal and highly conserved cellular response to stress. We describe here the effect of elevated temperature on the capacity of B cells to present antigen. Heat shock markedly affects the ability of these cells to process and present tetanus toxin to class 11-restrictedT cell clones. Inhibition of antigen presentation is due neither to a modification of antigen capture nor to a variation of major histocompatibility complex (MHC) class I1 molecule synthesis and cell surface expression. Stressed and nonstressed B cells are able to present peptides loaded at the cell surface with the same efficiency. Nevertheless, heat shock leads to an increase of antigen peptide generation in subcellular compartments; an enhancement of cathepsin B activity is also observed. These data suggest that such a stress induces a failure in the intracellular peptide loading onto MHC class I1 molecules.