Heat shock and cytokines modulate the expression of adhesion molecules on different human gastric-cancer cell lines
✍ Scribed by M.-C. Hsieh; C.-W. Wu; L.-H. Wu; W.-Y. Lui; F.-K. P'eng; C.-L. Yu
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- French
- Weight
- 496 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
In order to understand the expression and modulation of adhesion molecules (AMs) on the surface of different gastric cancers, we studied 4 gastric-cancer cell lines including SC-M I, KATO-Ill, AGS and AZ-521. The expression of E-cadherin, integrins (p I, p2 and 83). ICAMs (I and 2), and CD I I (a, b and c) on the cells was detected by flow cytometry. We found that E-cadherin was only expressed on SC-M I and KATO-Ill. CD29 (PI integrin) could be found in cells of all 4 lines. CD54 (ICAM-I) could not be detected in AZ-521. In contrast, CDl8 (p2 integrin), CD61 (p3 integrin), ICAM-2, CDI la, CDI I b and CD I I c were all absent from these cells. Heat-shock treatment (42.5"C, 60 min) enhanced the expression of E-cadherin, CD29 and CD54 on SC-M I, and of CD29 on AGS. In addition, TNF-a (50U/ml) and IL-l p (IOU/ml) modulated the expression of these AMs, like heat-shock treatment. The increment of these adhesion molecules caused by heat shock, TNF-cu and IL-I f3 stimulation on SC-MI was also confirmed by Western blot analysis. Functionally, these treatments increased the binding between normal human mononuclear cells and SC-MI cells. The heat-shock treatment could induce a significant amount of TNF-a and IL-I p release from SC-M I and KATO-Ill, but seemed irrelevant to the expression of AMs. These results suggest that limited adhesion molecules were expressed on the surface of different gastric cancer cells. Heat shock, IL-I p and TNF-a may selectively modulate the expression of these 3 molecules on some of the cells, and this is probably related to their antitumor effect.