Heart transplantation model as an immunological monitor

✍ Scribed by G. H. Müller

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 278 KB
• Volume: 11
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/micr.1920110208

✦ Synopsis

Microsurgery began over 25 years ago with standardized transplantation models. ' The first rat heart model was described in 1964.2 This, however, is ideal for only a few questions due to its considerable intervention in the recipi-mun; Sandoz) was administered once daily through a gastric tube in a dosage of 10 mgikg (experiment groups 111, IV, V, VI).

ent's anatomy. A more practicable model was described later by Ono and Lindsey.' With further development, we used two modified techniques for the heart model: 1 ) endto-end anastomosis and 2) end-to-side a n a ~t o m o s i s . ~ We would like to present the application of these techniques on some immunological experiments, depending on the prevailing problem. Rejection was tested with the heart model of a strong rat rejection model, in the sense of a first set and second set reaction. Furthermore, we tested whether this rejection reaction was dependent on position of implantation. Are grafts treated differently in the cardiovascular circulation system than in the portal system? Here, too, the heart model seemed adequate for testing.

Transplantation models were used to test immunological tolerance. We also used the heart model for such induction experiments, e.g., with cyclosporin A . Investigations of tolerance conditions show that organ differences obviously exist. We therefore tested whether animals that were tolerant to the kidney organ following kidney transplantation were also tolerant to a heart transplant from the same donor. We asked the same question of liver-transplanted recipients and implanted a donor-specific heart. The question of the specificity following such a tolerance induction was finally examined with third donor hearts. Both basic techniques for heart transplantations were applied in all experiments.