Headspace solid-phase microextraction for direct determination of volatile phenols in cider

✍ Scribed by Consuelo Pizarro; Nuria Pérez-del-Notario; José María González-Sáiz

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 298 KB
• Volume: 32
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900347

✦ Synopsis

Abstract

A headspace solid‐phase microextraction coupled to gas chromatography‐tandem mass spectrometry (GC‐MS/MS) method was optimised and validated for the determination of 4‐ethylguaiacol, 4‐ethylphenol, 4‐vinylguaiacol and 4‐vinylphenol, involved in the presence of Brett character, in ciders. The influence of different parameters on extraction efficiency (fibre coating, salt addition, exposure time, extraction temperature and sample volume/total volume ratio) was evaluated. Divinylbenzene/carboxen/PDMS was selected as extraction fibre and the other optimised parameters were as follows: 10 mL of cider, temperature 70°C, extraction time 60 min and addition of 0.4 g/mL of NaCl. The proposed method showed satisfactory linearity. The detection limits obtained were 0.01 μg/L for 4‐ethylguaiacol, 0.02 μg/L for 4‐ethylphenol, 0.08 μg/L for 4‐vinylguaiacol and 0.03 μg/L for 4‐vinylphenol. These detection limits were lower than those obtained in previous studies on the determination of volatile phenols in other alcoholic beverages. Good recoveries of over 95% were observed for all compounds, and the repeatability obtained was considered acceptable, ranging between 4 and 10%. To demonstrate the feasibility of the procedure, the method was applied to the analysis of commercial ciders. To our knowledge, this is the first time that the headspace solid‐phase microextraction procedure has been optimised to determine specifically the Brett character responsible compounds in cider.