Hb M Milwaukee: Direct detection of the β-globin gene mutation in three generations of an afflicted family

✍ Scribed by Renate Oehme; Elisabeth Kohne; E. Kleihauer; J. Horst

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 669 KB
• Volume: 64
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00292370

✦ Synopsis

Chromosomal DNA from three individuals with familial hemoglobin M (Hb M) Milwaukee was studied by restriction endonuclease analysis. The segregation of the mutant beta-globin gene could be followed through three generations by direct Sst I analysis at the gene level. Various restriction endonucleases were used to confirm the positions of Sst I sites in the delta-beta A- and delta-beta Mi-globin gene regions.