Harmine as a substitute for 33258 Hoechst in the FPG technique

✍ Scribed by M. G. Gutiérrez-Gonzálvez; M. J. Hazen; R. H. Espelosín

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 248 KB
• Volume: 89
• Category: Article
• ISSN: 1432-119X
• DOI: 10.1007/bf00489925

✦ Synopsis

We studied the effectiveness of harmine as a substitute for 33258 Hoechst in the fluorescence-plus-Giemsa technique, using Allium cepa chromosomes after 5-bromo-2'-deoxyuridine (BrdU) incorporation. Harmine showed a photosensitizing capacity which was somewhat higher than 33258 Hoechst and used half of the time established for the usual treatment.