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Haplodiploid gene expression in maize and its detection

✍ Scribed by C. Frova; M. Sari Gorla; E. Ottaviano; C. Pella

Publisher
Springer
Year
1983
Tongue
English
Weight
432 KB
Volume
21
Category
Article
ISSN
0006-2928

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✦ Synopsis

A method for the demonstration of the gametophytic origin of genetic variability in maize is described. For genes coding dimeric or multimeric enzymes, haploid expression can be demonstrated by means of translocations between A and B chromosomes (TB-A), which make it possible to obtain hyperploid pollen grains, partially diploid and heterozygous for electrophoretic mobility. Comparison of the electrophoretic pattern of this pollen type (three bands) and that of normal grains produced by a heterozygous F/S plant (two bands only) reveals haploid transcription of the monomeric forms. The procedure was tested on ADH-1 and used to demonstrate haploid expression for GOT-1. The data obtained suggest, moreover, that the reduction in male gamete transmission of duplications may be due to differences in pollen competitive ability rather than to processes affecting microspore maturation.

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