Handbook of Bacterial Adhesion: Principles, Methods, and Applications

✍ Scribed by Yuehuei H. An, Richard B. Dickinson, Ronald J. Doyle (auth.), Yuehuei H. An MD, Richard J. Friedman MD, FRSC(C) (eds.)

• Publisher: Humana Press
• Year: 2000
• Tongue: English
• Leaves: 646
• Edition: 1
• Category: Library

✦ Synopsis

In the Handbook of Bacterial Adhesion: Principles, Methods, and Applications, Yuehuei An, MD, and Richard Friedman, MD, assemble the first comprehensive treatment of bacterial adhesion, with major concentration on the powerful experimental methods for its study in biomaterials and host tissues. Written by acknowledged experts in the field, these detailed techniques facilitate the study of implant infections in a wide range of devices, including orthopedic prostheses, cardiovascular devices, cerebrospinal fluid shunts and extradural catheters, urological catheters and stents, oral and maxillofacial implants, and even contact lenses. Methods for the study of microbial adhesion to such tissues as respiratory and intestinal mucosa, the urinary tract, and teeth are also included, as are numerous diagrams, tables, line drawings, and photographs to illuminate and clarify the text.
Comprehensive and eminently practical, the Handbook of Bacterial Adhesion: Principles, Methods, and Applications offers biomedical scientists and bioengineering researchers an all-in-one experimental guide to today's best techniques for understanding the nature, mechanisms, and prevention of bacterial adhesion.

✦ Table of Contents

Front Matter....Pages i-xvi
Front Matter....Pages N1-N1
Mechanisms of Bacterial Adhesion and Pathogenesis of Implant and Tissue Infections....Pages 1-27
Molecular Basis of Bacterial Adhesion....Pages 29-41
Molecular Genetics of Bacterial Adhesion and Biofouling....Pages 43-51
Factors Influencing Bacterial Adhesion....Pages 53-72
Nonspecific Staphylococcus epidermidis Adhesion....Pages 73-90
Effects of Surface Roughness and Free Energy on Oral Bacterial Adhesion....Pages 91-102
Front Matter....Pages N3-N3
Basic Equipment and Microbiological Techniques for Studying Bacterial Adhesion....Pages 103-120
General Considerations for Studying Bacterial Adhesion to Biomaterials....Pages 121-132
Laboratory Culture and Analysis of Microbial Biofilms....Pages 133-169
Monitoring the Organization of Microbial Biofilm Communities....Pages 171-188
Models and Measurement of Bacterial Growth Rates on Polymers....Pages 189-202
Analysis of Gene Expression in Biofilm Bacteria....Pages 203-211
Front Matter....Pages N5-N5
Methods for Evaluating Attached Bacteria and Biofilms....Pages 213-233
Evaluating Adherent Bacteria and Biofilm Using Electron Microscopy....Pages 235-248
Confocal Laser Scanning Microscopy for Examination of Microbial Biofilms....Pages 249-257
Quantitation of Bacterial Adhesion to Biomaterials Using Radiolabeling Techniques....Pages 259-272
Evaluating Adherent Bacteria and Biofilm Using Biochemical and Immunochemical Methods....Pages 273-283
Evaluating Bacterial Adhesion Using Atomic Force Microscopy....Pages 285-296
Direct Measurement of Long-Range Interaction Forces Between a Single Bacterium and a Substrate Using an Optical Trap....Pages 297-306
Front Matter....Pages N7-N7
Staphylococcal Factors Involved in Adhesion and Biofilm Formation on Biomaterials....Pages 307-330
Front Matter....Pages N7-N7
Studying Bacterial Adhesion to Irregular or Porous Surfaces....Pages 331-343
Studying Bacterial Colonization of Tubular Medical Devices....Pages 345-352
Studying Plaque Biofilms on Various Dental Surfaces....Pages 353-370
Studying Bacterial Adhesion to Biliary Stents....Pages 371-388
Studying Bacterial Adhesion to Hydrogel Contact Lenses....Pages 389-396
In Vivo Models for Studying Staphylococcal Adhesion to Biomaterials....Pages 397-409
Front Matter....Pages N9-N9
Characterization of Staphylococcal Adhesins for Adherence to Host Tissues....Pages 411-444
Studying Bacterial Adhesion to Tooth Surfaces....Pages 445-455
Studying Bacterial Adhesion to Respiratory Mucosa....Pages 457-485
Studying Bacterial Adherence to Endothelial Cells....Pages 487-496
Studying Bacterial Adhesion to Gastric Epithelium....Pages 497-513
Studying Bacterial Adhesion in the Urinary Tract....Pages 515-525
Studying Candida albicans Adhesion....Pages 527-540
Studying Bacterial Adhesion to Cultured Cells....Pages 541-552
Front Matter....Pages N11-N11
Strategies for Preventing Group A Streptococcal Adhesion and Infection....Pages 553-579
Changing Material Surface Chemistry for Preventing Bacterial Adhesion....Pages 581-589
Antimicrobial Agent Incorporation for Preventing Bacterial Adhesion....Pages 591-598
Studying Bacterial Adhesion to Antibiotic Impregnated Polymethylmethacrylate....Pages 599-608
Macromolecule Surface Coating for Preventing Bacterial Adhesion....Pages 609-625
Back Matter....Pages 627-644

✦ Subjects

Microbiology