H2-Recycling system in mungbeanRhizobiumin relation to N2-fixation

✍ Scribed by Kamlesh Pahwa; Ramesh Chander Dogra

• Book ID: 104760679
• Publisher: Springer
• Year: 1981
• Tongue: English
• Weight: 406 KB
• Volume: 129
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00406467

✦ Synopsis

Thirty isolates of mungbean Rhizobium were tested for the presence of H2-recycling system. All the isolates were preliminary screened for detecting He-recycling system in free culture using triphenyltetrazolium chloride reduction as screening procedure. The isolates which reduced the dye rapidly at early stages of growth were found to recycle hydrogen both in vivo as well as in vitro. Nitrogen fixing efficiency of hydrogenase positive, hydrogenase negative isolates and Hup mutants was compared by green house experiments. There was 13-56 % increase in dry matter and 21-46% increase in total nitrogen of the plants inoculated with Hz-recycling isolates over the plants inoculated with non-recycling isolates. There was reduction in dry matter and total nitrogen content of the plants inoculated with Hupmutants as compared to plants inoculated with wild type strain. The per cent decrease due to inoculation with Hupmutants over wild type strain was 19-22 and 20-26 of dry weight and total nitrogen in plants, respectively.