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Growth yields and saturation constant ofDesulfovibrio vulgarisin chemostat culture

✍ Scribed by Regina Nethe-Jaenchen; Rudolf K. Thauer

Publisher: Springer
Year: 1984
Tongue: English
Weight: 509 KB
Volume: 137
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00414550

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✦ Synopsis

Desulfovibrio vulgaris (strain Marburg) was grown on H2 and sulfate as sole energy source in a chemostat limited by the sulfate supply. The biomass concentration and the sulfate concentration in the culture were determined as a function of the dilution rate. From the data a Ks (saturation constant) for sulfate of 10 ~tM, a #max of 0.23 h-1, and a ymax of 13 g/mol were calculated. The organism was also S 0 4 2grown in chemostat culture on H 2 and sulfite, H 2 and thiosulfate, and pyruvate (without sulfate). YsN]~-was found to be 35 g/tool, Ys%~2-36 g/mol, and ypmy, X 10 g/mol. The growth yields are discussed with respect to ATP gains in dissimilatory sulfate reduction.

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