Growth yields and growth rates ofDesulfovibrio vulgaris(Marburg) growing on hydrogen plus sulfate and hydrogen plus thiosulfate as the sole energy sources

✍ Scribed by Werner Badziong; Rudolf K. Thauer

• Book ID: 104758660
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 518 KB
• Volume: 117
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00402310

✦ Synopsis

A b s t r a c t . Desulfovibrio vulgaris (Marburg) was grown on H2 plus sulfate and H 2 plus thiosulfate as the sole energy sources and acetate plus CO2 as the sole carbon sources. Conditions are described under which the bacteria grew exponentially. Specific growth rates (/~) and molar growth yields (Y) at different pH were determined.

and Y were found to be strongly dependent on the pH. Highest growth rates and molar growth yields were observed for growth on H 2 plus sulfate at pH 6.5 (# = 0.15 h -1 ; Yso, ~-= 8.3 g-m o l -1) and for growth on H 2 plus thiosulfate at pH 6.8 (# = 0.21h-1; Ys2o~-= 16.9 g-tool-l).

The growth yields were found to increase with increasing growth rates: plots of 1 / Y versus 1//~ were linear. Via extrapolation to infinite growth rates a Y~s~ x of 12.2 g. m o l -1 and a Y~s2~X~ -of33.5g, m o l -1 was obtained.

The growth yield data are interpreted to indicate that dissimilatory sulfate reduction to sulfide is associated with a net synthesis of 1 mol of ATP and that near to 3 tool of ATP are formed during dissimilatory sulfite reduction to sulfide.