Growth Stimulation by Transfection of Intestinal Epithelial Cells with an Antisense Insulin-like Growth Factor-Binding Protein-2 Construct
β Scribed by M.R. Corkins; J.A. Vanderhoof; D.H. Slentz; R.G. Macdonald; J.H.Y. Park
- Book ID
- 115576796
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 406 KB
- Volume
- 211
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Insulinβlike growth factor binding proteinβ3 (IGFBPβ3) has been proposed to mediate the growth inhibitory effects of transforming growth factor (TGF)βΞ² in breast and prostate cancer cells. Both TGFβΞ² and exogenous IGFBPβ3 inhibit DNA synthesis in Mv1 mink lung epithelial cells (CCL64).
Using monolayer cultures of costal chondrocytes established from four week old Clun Forest lambs, we have demonstrated that, under serum free conditions the cells release three IGFBPs (32, 29 and 21 kDa) into the medium. The most abundant of these--the 32 kDa BP--was shown to be IGFBP-2 by Western b