Growth, sporulation and larvicidal activity ofBacillus sphaericus

✍ Scribed by A. Kalfon; I. Larget-Thiéry; J.-F. Charles; H. Barjac

• Book ID: 104787291
• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 960 KB
• Volume: 18
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00498040

✦ Synopsis

A new medium (MBS) for optimal sporulation of Bacillus sphaericus was defined. With the two main mosquito pathogenic strains grown in this medium, 1593-4 and 2297, highest cell and spore yields were obtained, concomitantly with an highest larvicidal activity against Culexpipiens. Study of both strains asporulated mutants showed a decrease in larvicidal power. After plasmid curing treatments, toxicity of strain 1593-4 did not decrease, neither toxic parasporal inclusion bodies of strain 2297 disappear.