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Growth Requirements of Murine Bone Marrow Macrophages in Serum-Free Cell Culture

✍ Scribed by Inge Flesch; E. Ferber


Book ID
118171105
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
743 KB
Volume
171
Category
Article
ISSN
0171-2985

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## Abstract A serum‐free medium for serial culture of baby hamster kidney cell line 21 (BHK‐21) as container‐adherent cells was developed. The medium is a 1:1 (v/v) mixture of Dulbecco's modified Eagle's medium and Ham's F‐12 medium supplemented with fibroblast growth factor, fibronectin, insulin,