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Growth requirements of endothelial cells in culture: Variations in serum and amino acid concentrations

✍ Scribed by Linda Gorman; L.Preston Mercer; Bernhard Hennig

Book ID
114286837
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
711 KB
Volume
12
Category
Article
ISSN
0899-9007

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## Abstract A serum‐free medium for serial culture of baby hamster kidney cell line 21 (BHK‐21) as container‐adherent cells was developed. The medium is a 1:1 (v/v) mixture of Dulbecco's modified Eagle's medium and Ham's F‐12 medium supplemented with fibroblast growth factor, fibronectin, insulin,