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Growth-regulatory mechanism of two human esophageal-cancer cell lines in protein-free conditions

✍ Scribed by K. Iihara; H. Shiozaki; K. Oku; H. Tahara; Y. Doki; H. Oka; T. Kadowaki; T. Iwazawa; M. Inouh; T. Mori

Publisher
John Wiley and Sons
Year
1993
Tongue
French
Weight
778 KB
Volume
55
Category
Article
ISSN
0020-7136

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✦ Synopsis

We investigated the growth-regulatory mechanism of 2 esophageal squatnous-cancer cell lines, TE2-NS and TE3-0s cells, both of which can grow stably in protein-free conditions in vitro. Protein-free conditioned media from TE2-NS and TE3-OS cells stimulated the growth of these cells. Exogenous epidermal growth factor (EGF), transforming growth factor-alpha (TGFa), insulin-like growth factor (IGF)-I and -11 enhanced cell proliferation by 2.2-to 3.8-fold in protein-free conditions, as compared with an untreated control. Receptor-binding assays showed that both TE2-NS and TE3-OS cells possessed a single class of high-affinity binding sites for IGF-I and 2 classes of binding sites for TGF-a, as confirmed on the cell membrane by immunochemistry. These results suggest that EGF, TGF-a and IGFs are candidates for the autocrine growth factor in cancer cells. The addition of inhibitory monoclonal antibodies against TGF-a and EGFR, but not those against either EGF or IGF-IR. significantly inhibited growth of the cells. lmmunocytochemical staining and ELISA of the conditioned media both confirmed the production of TGF-a protein, but not EGF protein, in these cell lines. The data for a protein-free culture system strongly suggested that TGF-a, but not EGF or IGF, is biologically important as an autocrine growth factor in the growth of these cell lines in vitro.

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