Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes
✍ Scribed by Josefine Enman; David Hodge; Kris Arvid Berglund; Ulrika Rova
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2012
- Tongue
- English
- Weight
- 85 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Abstract
BACKGROUND: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media.
RESULTS: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2–3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L^−1^. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L^−1^, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium.
CONCLUSION: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production. Copyright © 2012 Society of Chemical Industry