Growth-promoting properties of electrophoretic fractions of chicken embryo extract

✍ Scribed by J.O. Ely

• Publisher: Elsevier Science
• Year: 1954
• Tongue: English
• Weight: 353 KB
• Volume: 258
• Category: Article
• ISSN: 0016-0032
• DOI: 10.1016/0016-0032(54)90026-5

✦ Synopsis

Embryo extract has been recognized for a long time as an excellent source of the growth substances necessary for the culture of mammalian cells Zn o&o.

Many attempts have been made to separate the growth factors but no clear-cut separation has been accomplished.

Various fractions of chicken embryo extract were made by Hueper et al (1) by chemical means, no one of which contained the growth factors to the exclusion of the others. By differential centrifugation and precipitation with streptomycin, Kutsky (2) obtained a non-dialyzable fraction from chicken embryo extract which possessed most of the growth-promoting activity.

Chicken embryo extract has been fractionated in the Tiselius electrophoresis apparatus and the several fractions compared in their growthpromoting powers.