✦ LIBER ✦
Growth ofEscherichia coliB/r/l in a semi-continuous system designed for the synchronization of cell division
✍ Scribed by David Edward Buckley; Gerasimos Demetriou Anagnostopoulos
- Book ID
- 104778140
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 375 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
✦ Synopsis
Escherichia coli B/r/1 was grown under conditions of periodic feeding. Glucose, the only carbon source, was supplied at intervals longer than the generation time of the organism. Thus, each period of glucose availability was followed by a period of depletion. This process gave rise to two synchronous populations, one dividing shortly after a new supply of fresh medium and the other dividing at a later stage within the feeding cycle. Thymine incorporation experiments suggested that the double population emerged as the result of a discriminatory blockage of DNA replication.