Growth of yeast on n-alkanes. II. Batch experiments

✍ Scribed by A. H. M. Verkooyen; K. Rietema

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 742 KB
• Volume: 22
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260220311

✦ Synopsis

Abstract

In this paper the results of the Monte Carlo simulations as described in an earlier paper are compared with those of batch experiments. A number of batch experiments were carried out at a low inoculation rate so that only a fraction of the oil drops were inoculated. Under these conditions the effect of the segregation of the oil phase is more clearly demonstrated. Special attention is paid to the preparation of actively growing yeast cells with which the cultures is inoculated. Also a method is developed to estimate the amount of actively growing cells with which the culture is inoculated. The other parameters necessary for the Monte Carlo simulation are measured in separate experiments: the maximum growth rate of the cells, oil drop size, and the drop parameters. Finally the growth curves (measured in the batch experiments) are compared with those calculated with the Monte Carlo procedure. A good agreement is found.