GROWTH OF MDA-MB-231 CELL LINE: DIFFERENT EFFECTS OF TGF-β1, EGF AND ESTRADIOL DEPENDING ON THE LENGTH OF EXPOSURE

✍ Scribed by Cristina Mur; Pere A. Martı́nez-Carpio; Marı́a E. Fernández-Montolı́; Josep M. Ramon; Pilar Rosel; Miguel A. Navarro

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 215 KB
• Volume: 22
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1998.0306

✦ Synopsis

The human cell line MDA-MB-231 is a prototype for the study of hormone-independent breast cancer. Modification of cell growth behaviour has been observed after treating these cells with growth factors. EGF is a typical stimulatory growth factor for many cell types, whereas transforming growth factor beta(1)(TGF-beta(1)) acts with inhibitory character. Here we observed cell growth inhibition after EGF as well as after TGF-beta(1)treatments. Nevertheless, in the 42-h experiments, EGF-treated cultures grew before (18 hours) respect to the TGF-beta(1)and E(2)-treated cultures (24 h), and in the 11-day experiments, EGF-treated cultures started growing (7 days) after TGF-beta(1)-treated cultures (5 days). Estradiol inhibited the proliferation of these cells only after several days of treatment.