In comparing ethanol production by free and Ca-alginate immobilized cultures of the thermotolerant yeast, Kluyverumyces marxianus IMB3, on glucose-containing media at 45ยฐC it was found that initial yields produced by the immobilized culture lagged behind those produced by cultures in free suspension
Growth of a thermotolerant ethanol-producing strain ofKluyveromyces marxianuson cellobiose containing media
โ Scribed by N. Barron; R. Marchant; L. McHale; A. P. McHale
- Publisher
- Springer Netherlands
- Year
- 1994
- Tongue
- English
- Weight
- 319 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0141-5492
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โฆ Synopsis
The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at_45ยฐC. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable funga113-glucosidase. During active growth of the organism on cellobiose-containing media, 13-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added [3-glucosidase which results in increased substrate access to the biocatalytic unit.
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