Growth monitoring and control in complex medium: A case study employing fed-batch penicillin fermentation and computer-aided on-line mass balancing
✍ Scribed by Duen-Gang Mou; Charles L. Cooney
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 492 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
To broaden the practicality of on-line growth monitoring and control, its application in fedbatch penicillin fermentation using high corn steep liquor (CSL) concentration (53 g/L) is demonstrated. By employing a calculation method that considers the vagaries of CSL consumption, overall and instantaneous carbon-balancing equations are successfully used to calculate, on-line, the cell concentration and instantaneous specific growth rate in the penicillin production phase. As a consequence, these equations, together with a feedback control strategy, enable the computer control of glucose feed and maintenance of the preselected production-phase growth rate with error less than 0.002 h-'.
'Medium pH was adjusted to 6.5 with 6 N sodium hydroxide solution after sterilization.