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Growth kinetics of Dioscorea deltoidea and Catharanthus roseus in batch culture

✍ Scribed by D. Drapeau; H. W. Blanch; C. R. Wilke

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 752 KB
Volume: 28
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.260281014

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✦ Synopsis

Sugar-limited batch growth of Dioscorea deltoidea and Catharanthus roseus plant cell cultures was studied in a 14-L stirred tank fermentor. With dissolved oxygen concentration monitored and maintained at nonlimiting levels, growth rates and ratios of dry weight to fresh weight were found to be strongly influenced by sugar concentration. Linear relationships between respiration rate and growth rate were observed, and respiration rate was found to drop to a maintenance level after sugar had been fully depleted from the medium. Diosgenin biosynthesis by D. deltoidea was shown to-be independent of growth rate. Ajmalicine biosynthesis in C. roseus was negligible during sugar-limited growth but was induced by inoculation into an 80-g/L glucose solution.

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