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Growth-inhibitory effect of TGF-B on human fetal adrenal cells in primary monolayer culture

✍ Scribed by Lise Riopel; Charlotte L. Branchaud; Cynthia G. Goodyer; Vinita Adkar; Yves Lefebvre

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 560 KB
Volume: 140
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041400207

No coin nor oath required. For personal study only.

✦ Synopsis

We examined the effects of transforming-growth factor-B (TGF-B) on growth ([3H]-thymidine uptake) and function (dehydroepiandrosterone sulfate [DHAS] and cortisol production) of human fetal zone adrenal cells. Results indicate that TGF-B significantly inhibits, in a dose-related manner, both basal and epidermal growth factor (EGF)-stimulated cell growth: IC50 = 0.1-0.25 ng/ml. EGF is ineffective in overcoming the inhibitory effect of TGF-B, suggesting a noncompetitive antagonism between the two factors. Also, the inhibitory effect of TGF-B is additive to that of adrenocorticotropic hormone (ACTH). On the other hand, TGF-B (1 ng/ml) does not significantly change basal or ACTH-stimulated DHAS or cortisol secretion. We conclude that, unlike its effect on other steroid-producing cells, TGF-B inhibits growth of fetal zone cells and does not appear to have a significant inhibitory effect on steroidogenesis.

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