✦ LIBER ✦

Growth inhibition of breast cancer cells induced by exogenous ATP

✍ Scribed by Dr. Ben Spungin; Ilan Friedberg

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 780 KB
Volume: 157
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041570309

No coin nor oath required. For personal study only.

✦ Synopsis

Addition of ATP (>0.1 mM) to cultures of human breast cancer T47D cells resulted in an inhibition of cell proliferation. The inhibition was found to be specific for ATP, and dependent on its concentration. Growth inhibition continued for at least three days, although ATP and its hydrolysis products were metabolized within one day. Conditioned medium from ATP-treated cultures (CM+) was found to inhibit the growth of cells that were not exposed to ATP. This is an indication that extracellular factors, besides ATP, are involved in the inhibition process. The inhibition was maintained after dialysis of the CM+, using an 8 kDa cut-off membrane. Conditioned medium from untreated cultures (CM-), however, only slightly affected cell growth. The data suggest that the CM+-induced cell growth inhibition is mediated by an ATP-activated growth inhibiting factor. Flow microfluorometry and thymidine incorporation experiments have shown that the growth arrest is mainly due to the elongation of the S-phase of the cell cycle. 0 1993 Wiley-Liss, Inc.

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