✦ LIBER ✦

Growth factors that stimulate human granulocyte—macrophage colony formation produced by the cell line CM-S

✍ Scribed by Luisa Bertolini; Richard H. Butler; Roberto P. Revoltella

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 651 KB
Volume: 119
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041190111

No coin nor oath required. For personal study only.

✦ Synopsis

CM-S is an autonomous cell line of human hemopoietic precursor cells inducible to monocyte-macrophage differentiation in response to appropriate inducing agents. CM-S cells produce factors that stimulate their own growth and proliferation, and are also capable of stimulating clonal proliferation of human, but not mouse, monocytic and granulocytic bone marrow progenitor cells in viscous medium. Preliminary purification steps have demonstrated at least two species, one of which (MW 30,000-50,000) retains both these activities, while the other (MW <10,000) apparently retains only the autostimulatory activity. CM-S cells could thus be a useful source for the purification of human colony stimulating factors (CSFs). CM-S cells also respond to factors present in human placenta conditioned medium, known to contain human CSF. This suggests that CM-S cells could provide a homogeneous target cell population for testing CSFs from other h u m a n sources.

📜 SIMILAR VOLUMES

Role of Na+/H+ exchange in the granulocy

Role of Na+/H+ exchange in the granulocyte-macrophage colony-stimulating factor-dependent growth of a leukemic cell line

✍ Daniele Caracciolo; Antonella Pannocchia; Silvia Treves; Dario Ghigo; Eugenio Ga 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 764 KB

The growth of the human leukemia cell line AML-193 in a serum-free medium is strictly dependent o n the presence of the cytokine granulocyte-macrophage COIL ony-stimulating factor (GM-CSF), which is one of the major regulators of the myelomonocytic lineage. At present, little is known about the mech

Human lung mast cells are enriched in th

Human lung mast cells are enriched in the capacity to produce granulocyte-macrophage colony-stimulating factor in response to IgE-dependent stimulation

✍ Yoshimichi Okayama; Hiroyuki Kobayashi; Leonie K Ashman; Kunio Dobashi; Tsugio N 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 246 KB 👁 1 views

A combination of stem cell factor and gr

A combination of stem cell factor and granulocyte colony-stimulating factor enhances the growth of human progenitor B cells supported by murine stromal cell line MS-5

✍ Masamichi Nishihara; Yuka Wada; Kazuo Ogami; Yasuhiro Ebihara; Takefumi Ishii; K 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 224 KB 👁 2 views

Expression of the transforming growth fa

Expression of the transforming growth factor-α gene by human eosinophils is regulated by interleukin-3, interleukin-5, and granulocyte-macrophage colony-stimulating factor

✍ Marion A. Brach; Claudia Sott; Michael Kiehntopf; Friedhelm Herrmann 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 608 KB

Growth regulation of the AML-193 leukemi

Growth regulation of the AML-193 leukemic cell line: Evidence for autocrine production of granulocyte-macrophage colony-stimulating factor (GM-CSF), and inhibition of GM-CSF-dependent cell proliferation by interleukin-1 (IL-1) and tumor necrosis factor (tnfα)

✍ Vincent Kindler; John Shields; Dominique Ayer; Gonzalo J. Mazzei 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 583 KB

The human leukemic cell line AML-193 was tested for its proliferative response to endogenously produced autocrine factors and to a variety of cytokines and colony-stimulating factors. Cells grown in the absence of GM-CSF incorporated tritiated thymidine, and this was partially reversed by adding neu