โ Scribed by Stevens, S. E. ;Baalen, C.
No coin nor oath required. For personal study only.
After exposure of cells to N-methyl-N'-nitro-N-nitrosoguanidine a series of stable mutants of the marine coccoid blue-green alga, Agmenellum quadruplicatum, were isolated.
Two of these mutants were blocked at nitrate reductase and required nitrite or ammonium ion for growth.
Four of the mutants were blocked at nitrite reductase, required NH4+ for growth but had a functional nitrate reductase. These mutants readily reduced NO a-to NO2-.
Two of the mutants had altered phyeocyanin/chlorophyll a ratios, together with slightly impaired growth rates.
Mutant AQ-19 responded to casamino acids and possibly represents an amino acid auxotroph.
๐ SIMILAR VOLUMES
U.V. induced mutants of nitrogen-fixing blue-green algae appear to have substantial promise for studies in biochemical mechanism of nitrogen-fixation. Many types of U.V. induced morphological, physiological and biochemical mutant strains have been isolated in Chlamydomonas
1. Growth requirements of blue-green algae containing only the c-phycocyanin -[-chlorophyll a pigment system have been studied under blue light (380--540 nm) which approximates light conditions existing in subsurface waters in nature. 2. While a few species were capable of very slow photosynthetic