Growth changes in mammalian cell cultures by plastic additives. (effect of triethyl citrate)

✍ Scribed by Rosenbluth, S. A. ;Guess, W. L. ;Autian, J.

• Publisher: John Wiley and Sons
• Year: 1967
• Tongue: English
• Weight: 627 KB
• Volume: 1
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820010203

✦ Synopsis

Inhibition of cultured mammalian cell growth was used to examine the biologicd activity of triethyl citrate, a common plasticizer for poly(viny1 chloride). Cell populations were determined by a simple extraction procedure and measuremelit of iiucleic acid content (total purines and pyrimidines) by its absorbance at 268 mp. Triethyl citrate demonstrated strikingly different dose-effect characteristics from its theoretical hydrolysis product,s (et,hanol and citric acid). The inhibitory effects of sodium citrate were reversed by calcium chloride while the action of triethyl citrate underwent, little or no change under similar conditions. Triethyl citrate did not appear to produce resistant cell strains in culture but did seem to be degraded with time. Inhibitory action of triethyl citrate was shown to be independent of iiioculum size within the ranges utilized in the study. The procedures described appear to have a number of advant,ages for the biological or toxicological evaluation of ingredients which might be added to a plastic formulation or to contamiiiates which might enter the plastic during manufacture of the item or device.