Growth and survival ofPseudomonas cepaciaDBO1(pRO101) in soil amended with 2,4-dichlorophenoxyacetic acid

✍ Scribed by Carsten Suhr Jacobsen; Jens Christian Pedersen

• Publisher: Springer Netherlands
• Year: 1992
• Tongue: English
• Weight: 571 KB
• Volume: 2
• Category: Article
• ISSN: 0923-9820
• DOI: 10.1007/bf00114556

✦ Synopsis

The 2,4-dichlorophenoxyacetic acid (2,4-D) degrading pseudomonad, Pseudomonas cepacia DBOI(pRO101), was inoculated at approximately 107 CFU/g into sterile and non-sterile soil amended with 0, 5 or 500 ppm 2,4-D and the survival of the strain was studied for a period of 44 days. In general, the strain survived best in sterile soil. When the sterile soil was amended with 2,4-D, the strain survived at a significantly higher level than in non-amended sterile soil. In non-sterile soil either non-amended or amended with 5 ppm 2,4-D the strain died out, whereas with 500 ppm 2,4-D the strain only declined one order of magnitude through the 44 days.

The influence of 0, 0.06, 12 and 600 ppm 2,4-D on short-term (48 h) survival of P. cepacia DBOI(pRO101) inoculated to a level of 6 x 10 4, 6 x 106 or i x 108 CFU/g soil was studied in non-sterile soil. Both inoculum level and 2,4-D concentration were found to have a positive influence on numbers of P. cepacia DBOI(pRO101).

At 600 ppm 2,4-D growth was significant irrespective of the inoculation level, and at 12 ppm growth was stimulated at the two lowest inocula levels.

P. cepacia DBOI(pRO101) was able to survive for 15 months in sterile buffers kept at room temperature.

During this starvation, cells shrunk to about one third the volume of exponentially growing cells.