Growth and metabolism of human tumor kidney cells on galactose and glucose

✍ Scribed by A. Wagner; A. Marc; J. M. Engasser; A. Einsele

• Book ID: 104635930
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 517 KB
• Volume: 7
• Category: Article
• ISSN: 0920-9069
• DOI: 10.1007/bf00135633

✦ Synopsis

The culture kinetics of human tumor kidney cells (TCL 598) grown on microcarriers are compared with media initially supplemented with either glucose alone or a mixture of galactose and glucose. Growth rates and maximal cell densities are similar, but cellular death is much slower in galactose than in glucose. Galactose is metabolized at a much slower specific rate than glucose. Cells grown in the galactose medium show a different pattern of lactate and pyruvate metabolism compared to cells grown in the glucose medium. Growth with galactose also favours oxidation of glutamine to alanine.