Growth and macromolecular content of the dimorphic fungusAureobasidium pullulansand the effect of hydroxyurea and other inhibitors

✍ Scribed by Maria J. Sevilla; M. Dolores Moragues; Federico Uruburu

• Book ID: 104753969
• Publisher: Springer Netherlands
• Year: 1988
• Tongue: English
• Weight: 718 KB
• Volume: 54
• Category: Article
• ISSN: 0003-6072
• DOI: 10.1007/bf00393959

✦ Synopsis

The growth kinetics and the macromolecular content of the yeast and ethanol-induced hyphal forms of A ureobasidium pullulans were studied. During the morphological transition from yeasts to hyphae, both the protein and RNA content decreased significantly, the mycelial form containing only 76% of the amount of protein in the yeasts, and 38% of the RNA. The DNA was the only component tested whose level increased during the transition. Among several compounds inhibiting macromolecular synthesis, only hydroxyurea showed a remarkable effect on the morphology ofA. pullulans, inducing the mycelial morphology. The macromolecular composition of hydroxyurea-treated cultures changed with time in a way similar to that of the ethanol-Tween 80-ammonia medium, and to that of carbon-starved cultures, without ethanol or glucose.