Growth and energy metabolism in aerobic fed-batch cultures of Saccharomyces cerevisiae: Simulation and model verification

✍ Scribed by Hop Thi Bich Pham; Gen Larsson; Sven-Olof Enfors

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 219 KB
• Volume: 60
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19981120)60:4<474::aid-bit9>3.0.co;2-j

✦ Synopsis

A kinetic model of overflow metabolism in Saccharomyces cerevisiae was used for simulation of aero- bic fed-batch cultivations. An inhibitory effect of ethanol on the maximum respiration of the yeast was observed in the experiments and included in the model. The model predicts respiration, biomass, and ethanol formation and the subsequent ethanol consumption, and was experimentally validated in fed-batch cultivations. Oscillating sugar feed with resulting oscillating carbon dioxide production did not influence the maximum respiration rate, which indicates that the pyruvate dehydrogenase complex is not involved as a bottleneck causing aerobic ethanol formation.