𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Growth and biochemical characteristics of a detachment variant of CHO cells

✍ Scribed by Alan G. Atherly; Benjamin J. Barnhart; Paul M. Kraemer

Book ID
102879654
Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
813 KB
Volume
90
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

A variant subline of Chinese hamster cells (line CHO) was isolated that had increased resistance to detachment from the substratum. Comparisons between parental and variant cells of the complex carbohydrates liberated during trypsin detachment showed that the variant cells synthesized little or no hyaluronic acid. These cells also had reduced amounts of other complex carbohydrates in the cell periphery. However, parental and variant cells did not differ in morphology, growth control, or cyclic AMP concentration. Profound changes in the physical and chemical nature of the cell periphery, in themselves, evidently are insufficient to cause changes in many aspects of cell behavior.

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