✍ Scribed by Dr. B. Röber; K. H. Riemay; A. Hilpert
No coin nor oath required. For personal study only.
The autolytic process observed in fungi has been divided into three different phases A 1, A 2 , A 3. Each of which is characterized by specific metabolic evants. Recently, we characterized these phnses by means of "V-D-glucose. I n this communicatiori we report on the uptake and conversion of llC-labelled amino acids. These amino acids were rapidly taken up by the fungal mycelium and incorporated into cellular pools. During autolysis phase A 1 these amino acids were still used for protein synthesis. The autolytic process resembled a turnover process during this first phase. At this time the mycelium still exhibited high metabolic activity. The second phase, A 2 was characterized by a rapid intracellular degradation of compartmental material and accompanied by oxidation of the products formed. This was reflected by a n increasing 14C0, production. During phase A 3 products of intracellular degradation and possibly compounds from autolysing hyphae were still taken up by intact, i. e. living compartments of the hyphae and converted to heat and CO,. Almost no radioactive material was released into the medium during the last two phases. Metabolically active compartments or parts of the hyphae were present during the whole process of autolysis of fungal batch culture, .i e. even a t the end of phase A 3.
Culture conditions: Cultures of strain m 359 of Hyponzyces ochraceus (PERs.) TUL. (Weimar Collec-
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