Green Polymers from Geobacillus thermodenitrificans DSM465 – Candidates for Molecularly Imprinted Materials
✍ Scribed by Masakazu Yoshikawa; Kensuke Kawamura; Akinori Ejima; Takashi Aoki; Shinichi Sakurai; Kyoko Hayashi; Kunihiko Watanabe
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 154 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
Summary: Novel molecular recognition materials were prepared from water soluble proteins from thermophile G. thermodenitrificans DSM465 by an alternative molecular imprinting method. Water soluble proteins from G. thermodenitrificans DSM465 were converted into the molecularly imprinted materials by adopting 9‐EA as a print molecule. The molecularly imprinted protein membranes recognized As in preference to Gs. The adsorption isotherms led to the conclusion that molecular recognition sites toward As were constructed by the presence of 9‐EA during the membrane preparation process. The affinity constant between As and the molecular recognition site thus constructed was determined to be 1.75 × 10^5^ mol^−1^ dm^3^. The results obtained in the present study suggest that water soluble proteins from G. thermodenitrificans DSM465 is one of environmentally‐friendly ‘green’ polymers to be converted into molecular recognition materials by applying an alternative molecular imprinting method.
Adsorption isotherms of As and Gs in the 9‐EA molecularly imprinted protein and the control non‐imprinted membranes. (a): As in the 9‐EA molecularly imprinted membrane. (b): Gs in the 9‐EA molecularly imprinted membrane. (c): As in the control non‐imprinted membrane. (d): Gs in the control non‐imprinted membrane.
magnified imageAdsorption isotherms of As and Gs in the 9‐EA molecularly imprinted protein and the control non‐imprinted membranes. (a): As in the 9‐EA molecularly imprinted membrane. (b): Gs in the 9‐EA molecularly imprinted membrane. (c): As in the control non‐imprinted membrane. (d): Gs in the control non‐imprinted membrane.