Green Fluorescent Protein: Properties, Applications, and Protocols, Volume 47, Second Edition

• Year: 2005
• Tongue: English
• Leaves: 471
• Series: Methods of Biochemical Analysis
• Category: Library

✦ Synopsis

Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications.

This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.Content:
Chapter 1 Discovery of Green Fluorescent Protein (pages 1–13): Osamu Shimomura
Chapter 2 Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems (pages 15–38): J.Woodland Hastings and James G. Morin
Chapter 3 Biochemical and Physical Properties of Green Fluorescent Protein (pages 39–65): William W. Ward
Chapter 4 The Three?Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design (pages 67–82): George N. Phillips Jr.
Chapter 5 Molecular Biology and Mutation of Green Fluorescent Protein (pages 83–120): David A. Zacharias and Roger Y. Tsien
Chapter 6 Discovery and Properties of GFP?Like Proteins from Nonbioluminescent Anthozoa (pages 121–138): Konstantin A. Lukyanov, Dmitry M. Chudakov, Arkady F. Fradkov, Yulii A. Labas, Mikhail V. Matz and Sergey Lukyanov
Chapter 7 Evolution of Function and Color in GFP?Like Proteins (pages 139–161): Mikhail V. Matz, Yulii A. Labas and Juan Ugalde
Chapter 8 The Uses of Green Fluorescent Protein in Prokaryotes (pages 163–178): Raphael H. Valdivia, Brendan P. Cormack and Stanley Falkow
Chapter 9 The Uses of Green Fluorescent Protein in Yeasts (pages 179–201): Amy L. Hitchcock, Jason A. Kahana and Pamela A. Silver
Chapter 10 Uses of GFP in Caenorhabditis Elegans (pages 203–226): Oliver Hobert and Paula Loria
Chapter 11 Green Fluorescent Protein Applications in Drosophila (pages 227–257): Tulle Hazelrigg and Jennifer H. Mansfield
Chapter 12 The Uses of Green Fluorescent Protein in Plants (pages 259–284): Jim Haseloff and Kirby R. Siemering
Chapter 13 Uses of GFP in Transgenic Vertebrates (pages 285–303): Sean Megason, Adam Amsterdam, Nancy Hopkins and Shuo Lin
Chapter 14 The Uses of Green Fluorescent Protein in Mammalian Cells (pages 305–337): Theresa H. Ward and Jennifer Lippincott?Schwartz
Chapter 15 Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescence Microscopy and Flow Cytometry (pages 339–359): Yu Fang, Olivier Dery, Michael Haugwitz, Pierre Turpin and Steven R. Kain
Chapter 16 Pharmaceutical Applications of GFP and RCFP (pages 361–389): Nicola Bevan and Stephen Rees
Chapter 17 Reassembled GFP: Detecting Protein–Protein Interactions and Protein Expression Patterns (pages 391–405): Thomas J. Magliery and Lynne Regan